promega，M-MLV Reverse Transcriptase，M1701

描述：上海起发签约promega

更新时间：2015-06-18

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详情介绍

M-MLV Reverse Transcriptase

· Protocol

· MSDS

· Components

M-MLV Reverse Transcriptase

· M-MLV Reverse Transcriptase

· M-MLV RT 5X Buffer

10,000u

200u/μl

M1701

QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl （pH 7.5 at 25°C）, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl （pH 8.3 at 25°C）, 7mM MgCl₂, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [³H]dTTP, 0.025mM oligo（dT）, 0.25mM poly（A） and 0.01% NP-40.

For product intended use please see Patents & Disclaimers tab.

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promega，M-MLV Reverse Transcriptase，M1701

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